Morphological analysis of lipid storage in intestinal cells.

<p>A) Worms were stained by culturing from the L1 stage to adult stage on a Nile Red-containing plate. Control worms (N2) show strong stains in intestinal cells. In the <i>T21D12.3</i> mutants, Nile Red stains intestinal cells weakly and the stain signals in body cavity outside of eggs seem higher than that of N2. Nile Red stains in the <i>T07A9.1</i> mutants were not changed so remarkable. B) Nile Red staining signals in intestinal cells were analyzed quantitatively by collecting signals from 6 worms (25 µm²). Asterisks indicate p<0.001 in Student's t-test. Quantitative analysis showed a significant difference between N2 and <i>T21D12.3</i> mutants but not between N2 and <i>T07A9.1</i> mutants.