BMP-7 does not inhibit TGFβ₁ induced gene expression in fibroblasts.

<p>Primary human lung fibroblasts (n = 3) were serum starved for 24 hours before incubation with rhBMP-7 at 10 or 50 ng/ml for one hour and then stimulated with rhTGFβ₁ for 24 hours. Relative changes in mRNA expression of (A) procollagen-1, (B) αSMA, (C) CTGF and (D) TGFβ₁ were analyzed using real-time RT-PCR. Protein expression of αSMA and phosphoSMAD 1,5,8, was (E) visualized and (F) quantitated to β-tubulin by Western blot analysis. * indicates p<0.05 significant difference in comparison to unstimulated cells.