sICAM-1 levels in culture supernatants from immature (a) and mature oocytes (b).

<p>Immature oocytes were analysed individually for morphological characteristics to differentiate them as Metaphase I (MI), germinal vescicle (GV) and degenerated (DEG). (a). Mature oocytes were subdivided on the basis of the first polar body and cytoplasm characteristic in Grade 1, 2 and 3 (b). Preparation of oocyte supernatants was performed as described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003970#pone-0003970-g002" target="_blank">Figure 2</a>. * Student t Test.