Localization accuracy obtained from mKikGR molecules.

<p>(A) Fluorescence intensity trajectory of a single mKikGR molecule. The trajectory was constructed from single-molecule images of the red mKikGR (100 ms integration, 1 Hz). (B) Centroid positions obtained from single-molecule images of the red mKikGR. (C) Relationship between the localization accuracy and the total number of photons obtained from individual red mKikGR molecules (circle). The images were recorded with 568 nm excitation at the power of 13 (black circle), 33 (red circle), 65 (yellow circle), and 130 (blue circle) W cm⁻². The open circle shows the relationship between the localization accuracy and the total number of photons obtained from individual qdots. The solid line shows theoretically calculated localization accuracy using equation 1.