Inhibition of protein synthesis is sufficient to activate apoptosis and necroptosis in U937 cells.

<p>(A). Cycloheximide (5 µg ml⁻¹) induce caspase activity in U937 cells in a manner similar to both Diphtheria toxin and DT-GMCSF as determined using a caspase 3/7 luminogenic assay (mean+/−SEM from 3 replicates). (B). Cell viability of U937 cells in response to cycloheximide (5 µg ml⁻¹) in the presence of zVADfmk (50 µM) and/or Necrostatin-1 (50 µM) was measured using MTS assays (mean+/−SEM from 4 replicates). (C). MTS assays were used to measure the ability of Geldanamycin (0.5 µM) and zVADfmk (25 µM) to protect against cycloheximide (5 µg ml⁻¹) induced cell death in U937 cells (mean+/−SEM from 3 replicates) (* P<0.05) (**P<0.01) (#P<0.005) (##P<0.001).