Possible mechanisms underlying altered testosterone/estrogen ratio in <i>GPRC6A</i>^−/− male mice.

<p>(A and B) RT-PCR and real-time RT-PCR analysis of <i>androgen receptor</i> (<i>AR</i>) expression. <i>AR</i> expression in testis (Te) and bone marrow (BM) was not different between <i>GPRC6A^+/+</i> and <i>GPRC6A</i>^−/− male mice. (C) Real time RT-PCR analysis of <i>aromatase</i> expression in testis. (D and E) Comparison of the aromatase protein expression in testis from <i>GPRC6A^+/+</i> and <i>GPRC6A</i>^−/− male mice. Small increments in aromatase protein expression were observed in <i>GPRC6A</i>^−/− male mice by Western blot analysis (D) that was localized by immunhistochemistry (E) to Leydig cells (L, arrow head) and to a lesser degree in sertoli cells (SC), and spermatogonia (SG) (respectively indicated by arrows). (F and G) Real time RT-PCR analysis of <i>Cyp17</i> and <i>Sult1e1</i> expression in testis. (H and I) RT-PCR and real-time RT-PCR analysis of <i>GnRH</i> expression in brain.