Correlation of taxonomic assignments for human gut sequences based on full-length SSU rRNA, V3 and V6 variable regions.

<p>At each taxonomic level, the number of sequences from a particular taxon using one sequencing strategy (e.g., V6 tags) is plotted against the number of sequences from that same taxon using a second sequencing strategy (e.g., full-length SSU rRNA genes). For instance, classifying to the genus level, the Clostridial genus <i>Ruminococcus</i> occurred 186 times in the full-length SSU rRNA sequences and 19,332 times in the V6 tags. The order Clostridiales occurred 3,613 times in the full-length sequences, and 217,482 times in the V6 tags. <i>Note</i>: correlations are linear, although the axes use log scales for clarity. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000255#pgen-1000255-g003" target="_blank">Figure 3A</a> compares V6 tags with full-length sequences, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000255#pgen-1000255-g003" target="_blank">Figure 3B</a> compares V3 tags with full-length sequences, and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000255#pgen-1000255-g003" target="_blank">Figure 3C</a> compares V6 and V3 tags.