Similar amounts of organization at the <i>eve</i> stripe 4+6 enhancers of <i>Drosophila</i> and <i>Themira</i>.

<p>(A) A dot plot alignment of the <i>eve</i> stripe 4+6 enhancers of <i>Drosophila</i> and <i>Themira</i> at high stringency reveals highly conserved blocks of sequence, most of which are conserved in their basic order. (B) The blocks of alignment correspond to binding motifs for factors known to work at this enhancer: Tailless (pink), Hunchback (green), Knirps (orange), and Giant (purple). The block marked with an asterisk corresponds to sites that have been recreated in the same location, see also (D). Consequently, these sites do not appear in the parallel homology plot in (A). (C) An example of one of the blocks of conserved sequence, block A, is depicted. (D) An example of a block of sequence (asterisk) that has apparently experienced compensatory mutations that have shifted binding sites enough to obliterate homology. Despite such sequence turnover, such signatures preserve the higher-order organization of the enhancer.