Spreading of LVS in intranasally infected mice.

<p>Mice were infected intra-nasally with 10⁵ CFU of LVS. (A) Bacteria were counted in BALF, lung tissue after lavage, MdLNs, spleens, and livers in animals sacrificed at the indicated time points after infection. Single cell suspensions derived from the various organs were diluted in PBS and plated for CFU counts. Each bar represents the average counts in 3 individual animals. Experiment was repeated 2 more times, resulting in similar observations. (B) Microscopy evaluation of MdLN colonization was performed on cryo-sections of MdLN, 72 hrs post infection with LVS. Sections were stained with rabbit anti-LVS hyperimmune serum and FITC-conjugated secondary antibody. One representative large infection focus is shown.