<p>A. Polyacrylamide gel electrophoresis of cleavage products of 5′end labeled mglB mRNA generated by RNase P holoenzyme and rEGSe or rEGSx libraries. The RNase P holoenzyme was reconstituted by 10 nM M1 RNA and 100 nM C5 protein. mglB mRNA cleavage products were separated on 8% polyacrylamide/7 M urea gels together with an alkali ladder and a partial RNase T1 digest of the same RNA. Arrows indicate positions selected for further analyses. The black filled triangles represent decreasing concentrations of rEGSs (1000-, 100-, and 10-fold molar excess to the mglB mRNA) incubated with RNase P holoenzyme. Lane OH represents the alkali ladder. Lanes T1 and M1+C5 represent reaction with RNase T1 and RNase P holoenzyme, respectively. The difference...
<p>Arrows indicate the 5’ ends of mRNA fragments, as identified by cloned 5’ RLM-RACE products, with...
International audienceThe T4 endoribonuclease RegB is involved in the inactivation of the phage earl...
<p>A) Histogram showing the percentage of reads mapping to a given position, out of the total number...
<p>A. Activities of stem EGS155 and EGS155R. B. Activities of stem EGS52, EGS73, and EGS148. If need...
<p>A. Activities of M1 EGS155. B. Activities of M1 EGS52, M1 EGS73, and M1 EGS148. The RNase P holoe...
<p>(A) Reactions performed with 5′-end-labeled RsaA alone (lane 3) or in the presence of an excess o...
125 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.Incomplete mercuration of mRN...
<p>A. Electrophoretic analysis of cleavage. Mg<sup>2+</sup>: RNA treated with 5 mM MgCl<sub>2</sub>....
<p>The cleavage sites and the number of sequenced clones corresponding to each site are indicated by...
RNase P is a ribozyme consisting of a catalytic RNA molecule and, depending on the organism, one or ...
<p>(A) Schematic representation of the two methods applied to map regions which are accessible to ol...
<p>Semi-random libraries of DNA 6-mers and RNase H hydrolysis of RNA-DNA hybrids were used to search...
<p>(<b>A</b>) Recombinant WT APE1 (lane 4), variant L104R (lane 5), and variant E126D (lane 6), each...
<p>A. Northern analysis of the mglB mRNA level in <i>E. coli</i>. The plasmid vector pKB283-Mlac wit...
The rate limiting step in RISC-mediated cleavage occurs before catalysis To analyze a potential infl...
<p>Arrows indicate the 5’ ends of mRNA fragments, as identified by cloned 5’ RLM-RACE products, with...
International audienceThe T4 endoribonuclease RegB is involved in the inactivation of the phage earl...
<p>A) Histogram showing the percentage of reads mapping to a given position, out of the total number...
<p>A. Activities of stem EGS155 and EGS155R. B. Activities of stem EGS52, EGS73, and EGS148. If need...
<p>A. Activities of M1 EGS155. B. Activities of M1 EGS52, M1 EGS73, and M1 EGS148. The RNase P holoe...
<p>(A) Reactions performed with 5′-end-labeled RsaA alone (lane 3) or in the presence of an excess o...
125 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.Incomplete mercuration of mRN...
<p>A. Electrophoretic analysis of cleavage. Mg<sup>2+</sup>: RNA treated with 5 mM MgCl<sub>2</sub>....
<p>The cleavage sites and the number of sequenced clones corresponding to each site are indicated by...
RNase P is a ribozyme consisting of a catalytic RNA molecule and, depending on the organism, one or ...
<p>(A) Schematic representation of the two methods applied to map regions which are accessible to ol...
<p>Semi-random libraries of DNA 6-mers and RNase H hydrolysis of RNA-DNA hybrids were used to search...
<p>(<b>A</b>) Recombinant WT APE1 (lane 4), variant L104R (lane 5), and variant E126D (lane 6), each...
<p>A. Northern analysis of the mglB mRNA level in <i>E. coli</i>. The plasmid vector pKB283-Mlac wit...
The rate limiting step in RISC-mediated cleavage occurs before catalysis To analyze a potential infl...
<p>Arrows indicate the 5’ ends of mRNA fragments, as identified by cloned 5’ RLM-RACE products, with...
International audienceThe T4 endoribonuclease RegB is involved in the inactivation of the phage earl...
<p>A) Histogram showing the percentage of reads mapping to a given position, out of the total number...