The effects of FTY720 on accumulation of RTDC and LVS in the MdLN.

<p>Mice infected with 10⁵ LVS were treated with FTY720 diluted in carrier solution, or with carrier solution alone, as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000211#s4" target="_blank">Materials and Methods</a>. To examine DC trafficking, MdLNs were collected 48 hrs post infection and analyzed for RTDC representation (CD11b^high/autofluorescence^low) as described in the legend to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000211#ppat-1000211-g003" target="_blank">Figure 3</a>. An experiment was conducted on MdLN pools, each consisting of 6 organs. MdLNs were derived from infected mice treated with either carrier alone (A) or with FTY720 (B). Non-infected, FTY720-treated mice (C) served as controls. Bacterial colonization of the MdLN (D) was examined 48 hrs post infection in each one of the 6 individual FTY720- treated and 6 mock-treated mice, <i>P</i><0.01. Three independent experiments, each conducted with groups of six mice, exhibited similar correlation between RTDC and LVS recruitment.