Abnormal conidial morphology, appressorial formation, and hyphal branching in the Mgrac1 deletion mutant.

<p>(A) Differential interference contrast (DIC) microscopy of conidia cultured on an oatmeal agar plate at day 10 after incubation. BA = basal appendage where conidia attach to conidiophores. Bar = 20 µm. (B) Conidia incubated on the surface of artificial hydrophobic Gelbond films as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000202#s4" target="_blank">Materials and Methods</a>. Bar = 20 µm. (C) Branching patterns of mycelia on complete media plates at day 3 after incubation. Frequent branching occurs at the terminal mycelia of <i>ΔMgrac1-19</i>. Calcofluor staining of mycelia is used to show the distance of septa. Bar = 20 µm. (D) DAPI staining of mycelia to show the localization of nuclei. Bar = 20 µm.