cDNA microarray analysis of DNA vaccinated rats reveals upregulated type I IFN-regulated genes.

<p>(A) Changes in gene expression of immune system-related genes in pMOG-vaccinated <i>vs.</i> pCI-vaccinated control rats at 11 d after MOG_91–108 immunization (<i>n</i> = 6/group). Genes significantly differentially expressed as estimated using the SAM technique, which does not allow for calculations of SD or SEM. (B) Monocytes cultured with IFN-β upregulate IRF-7, CCR-7, Ly6C, TLR9 and IP-10 expression. Q-PCR analysis of the mean+/−SEM mRNA expression of relevant molecules in monocytes cultured with IL-4/GM-CSF or IFN-β/GM-CSF respectively (<i>n</i> = 4/group). (C) Mean TLR9, CCR-7 and IP-10 mRNA expression in splenocytes isolated from pMOG- or pCI-treated rats respectively 11 d after MOG_91–108 immunization (<i>n</i> = 5/group). All values are normalized to GAPDH mRNA. Bars represent mean+/−SEM. * <i>p</i><0.05.