Amiloride inhibits uPAR constitutive uptake in a dose-dependent manner.

<p>Panel A. Biotinylation experiments in HEK293-uPAR cells incubated in the absence or presence of amiloride. Lane 1: background, Lane 2: constitutively internalized uPAR, Lane 3: inhibition by amiloride. Panel B: Amiloride inhibits dose-dependently constitutive uPAR endocytosis measured by the cell cytometry assay. Panel C: 5 mM Amiloride specifically inhibits uPAR and 70kDa-Dextran endocytosis, but not Transferrin receptor endocytosis, as observed by the cell cytometry assay. Panel D: Amiloride specifically inhibits uPAR and CD59 but not LRP-1 endocytosis, as observed by the cell cytometry assay. Panel E: Amiloride inhibits uPAR but not uPA:PAI-1 dependent endocytosis, measured by the cell cytometry assay.