NF-κB activity during extinction session.

<p>A. Top diagram: experimental design. On day 1 the animals were trained with 15 trials (TR groups) or were located in the training apparatus but remained untrained (CT groups). On day two, the CT-TR groups were re-exposed to the training context. At different time points (indicated by arrows), animals of CT and TR pair of groups were anesthetized and nuclear extracts from the 20 central brains of each group were obtained. B. Representative EMSAs performed with nuclear extracts obtained from CT and TR groups at 5, 45 and 120 minutes of context re-exposure. The specific band is indicated with a filled arrow. C. NF-κB activity in TR group relative to CT. Mean±SEM of relative optic density (ROD) values of the specific NF-κB retarded band normalized to CT group media, obtained in four independent experiments. D, E and F. <u>NF-κB activity after memory reactivation.</u> D. Top diagram: experimental design. On day 1 the animals were trained with 15 trials (TR groups) or were located in the training apparatus but remained untrained (CT groups). On day two, the CT-TR groups were re-exposed for 5 minutes to the training context. At different time points after the re-exposure (indicated by arrows), animals of CT and TR pair of groups were anesthetized and nuclear extracts from the 20 central brains of each group were obtained. E. Representative EMSAs performed with nuclear extracts obtained from CT and TR groups at 5, 45 and 180 minutes after context re-exposure. The specific band is indicated with a filled arrow. F. NF-κB activity in TR group relative to CT. Mean±SEM of relative optic density (ROD) values of the specific NF-κB retarded band normalized to CT group media, obtained in four independent experiments. *, <i>p</i><0.05 in <i>t-</i>test.