The PPE41 and PE25/PPE41 complex proteins enhance CD4⁺ and CD8⁺ T cell activity.

<p>Splenocytes from naïve mice or mice immunized with each of the recombinant proteins (PE25, PPE41 and PE25/PPE41) were stimulated with different concentration of the corresponding proteins <i>in-vitro</i>. The intracellular IFN-γ secreted by CD4⁺ (a) and CD8⁺ T cells (b) specific to the PE25, PPE41 and the PE25/PPE41 complex proteins was measured by flow cytometry and summarized as % increase in different experimental groups (c). The population of CD4⁺ and CD8⁺ T cells specific to the recombinant proteins was measured by stimulating the splenocytes with the respective proteins for 24–36 hrs. The data shown represent one of at least three similar independent experiments.