Time course of cAMP production by Jurkat cells stimulated with EdTx, CT, CyaA, or forskolin.

<p>Jurkat T cells were stimulated for the indicated times with EdTx (10 nM EF+40 nM PA), CT (3 nM), CyaA (5 nM), or forskolin (25 µM). At the end of the incubations, the samples, which contained 10⁵ cells, were lysed and immediately subjected to competition ELISA in order to determine the amount of cAMP present. Adefovir dipivoxil, a specific inhibitor of EdTx, abolished cAMP synthesis in EdTx-treated cells (not shown). Note the different cAMP scales in the four panels. Experiments were not normalized to percentage values to provide the actual levels of cAMP. Data are the mean and standard deviation of four independent experiments for EdTx and three for CT, CyaA and forskolin, performed in triplicates.