Codon clustering within subsets of the <i>E. coli</i> ORFeome, separated by gene classification.

<p>2166 characterized genes from the <i>E. coli</i> ORFeome (dark line) are enriched in common codons as compared to 2325 genes annotated as unclassified, hypothetical, or unknown function (grey line). The median of each curve is denoted with an asterisk. %MinMax values were calculated using the codon usage frequencies from the entire ORFeome, with a sliding window of 18 codons.