Knockdown of MBP-1 in human foreskin fibroblasts induces cell proliferation.

<p>Panel A: Normal human foreskin fibroblasts were transfected with MBP-1 specific shRNA or control scrambled shRNA and selected with neomycin for 3 weeks. Colonies were pooled and examined for endogenous expression of MBP-1 by Western blot analysis using a specific antibody to MBP-1. Densitometric analysis of MBP-1/tubulin was performed using Image Quant Software (Amersham, ME). Panel B: Knockdown of MBP-1 inhibits HFF proliferation. Cells were plated after selection, and counted at different time points by trypan blue exclusion method. Panel C: MBP-1 knockdown in HFF results in accumulation of HFF at the G1 phase. Asynchronized HFF-control and HFF-MBPsi-4 were harvested fixed and stained with propidium iodide. DNA content was analyzed by flow cytometry. Results are represented as percent of cell population in G1, S, and G2/M phases of the cell cycle. Results are presented together with standard errors from three independent experiments.