M-CSF induces mononuclear phagocyte recruitment <i>in vivo</i>.

<p>A) Matrigel™ was resuspended with PBS <i>(PBS)</i> or rmM-CSF (100 ng/ml) <i>(M-CSF)</i> at 4°C overnight and then injected subcutaneously into mice. Matrigel™ was left <i>in situ</i> for 10 days and then harvested. The plugs were stained for mononuclear phagocytes using an anti-mouse F4/80 antibody. Brown staining represents F4/80(+) cells within the plug. Pictures shown were taken using a dissecting microscope (1.5× objective lens) <i>(left)</i> to display overall mononuclear phagocyte influx between conditions as well as an inverted microscope (20× objective lens) <i>(right)</i> to show detailed staining of these cells. B) The percent of F4/80(+) cells (brown stain) per plug was evaluated from 15 digital images captured randomly in a blinded manner. Adobe Photoshop histogram pixel analysis of the brown stain was used for quantification. This data represents three plugs per group.