MBP-1 knockdown in HFF modulates expression of cyclins.

<p>Panel A: Expression of cyclins in control and MBP-1 knockdown HFF. Lysates prepared from cells were subjected to Western blot analysis using indicated antibodies. Knockdown of MBP-1 enhanced cyclin D1 expression and cyclin E and reduced cyclin A expression in HFF-control. Panel B: Time-dependent elevation of cyclin D1 expression in MBP-1 knockdown HFF as compared to control HFF. Cells were synchronized by serum-starvation and lysates were prepared at different time points as indicated following serum stimulation. Cell lysates were examined for the expression of cyclin D1 by Western blot analysis using specific antibody. Protein load was normalized and determined level of cyclin D1 by densitometric analysis. Arbitrary unit was chosen to represent relative cyclin D1 expression. Results are presented together with standard errors from three independent experiments.