Loss of <i>de2f1</i> does not affect resistance to DNA damage and pupal developmental apoptosis in <i>wts</i> mutants.

<p>In all panels, clones were generated with the <i>ey</i>-FLP/FRT technique and mutant tissue is distinguished by the absence of GFP (green). (A–B) The pupal eye discs at 30 hr APF containing clones of <i>wts</i> mutant (A) and <i>de2f1 wts</i> double mutant (B) cells were stained with anti-Cleaved Caspase3 (C3) antibody (red) to detect apoptotic cells. In the pupal eye discs, developmentally regulated apoptosis is abundant in wild type cells (green) but is largely absent in <i>wts</i> mutant tissue (lack of green) and in <i>de2f1 wts</i> double mutant tissue indicating that <i>de2f1 wts</i> double mutant cells, like <i>wts</i> mutant cells, are protected from the cell death. (C) DNA damage induced apoptosis following irradiation was detected with anti-C3 antibody (red). There is an extensive apoptosis in wild type tissue. In contrast, <i>de2f1 wts</i> double mutant cells are protected from apoptosis after DNA damage. An example of <i>de2f1 wts</i> double mutant tissue is pointed by arrow. The morphogenetic furrow is marked by the arrowhead.