Pathological PrP^Sc conversion of PrP^C requires macropinocytosis.

<p>(A) N2aPK1 cells were exposed to 10⁻⁵ dilution of strain RML scrapie PrP^Sc-infected murine brain homogenate and increasing concentrations of the macropinocytosis inhibitor EIPA (25, 50 and 100 µM) for 48 hr. Infected cells were then grown on cover slips, blotted, digested with proteinase K (PK) and probed with anti-PrP antibodies (A) and Ponceau S staining (B).