The ability of rMtb-HBHA and PPD to induce the production of IFNγ was tested in the ELISPOT technique.

<p>The groups of patients with inactive TB (n = 5); active TB (n = 6), healthy young (n = 7) and middle-age (n = 8) vaccinated subjects and patients with isolation of so-called atypical mycobacteria (n = 4) and were enrolled. Comparison of the cell-mediated immunological responses to rMtb-HBHA was performed as the determination of the number of reactive cells per10⁶ lymphocytes (A) and as the measurement of IFNγ production activities expressed as arbitrary units (B). Cell-mediated immunological responses in healthy vaccinated individuals was studied by division by age into two groups (C). The correlation of rMtb-HBHA with the PPD ELISPOT reactivities expressed as the frequencies of reactive cells per10⁶ is presented in (D). Data are shown as individual reactivities; the horizontal bars represent arithmetic median values.