HCMV-specific effector and memory humoral and cellular immune responses following immunisation with Ad-gBCMVpoly vaccine.

<p><i>A,</i> HCMV-specific CD8+ T cell responses following immunisation with Ad-gBCMVpoly. These T cell responses were assessed using ELISPOT assays on day 10, 25 and 75 post immunisation. The results are expressed as Mean±SE of spot forming cells (SFC) per 10⁶ splenocytes. <i>B,</i> gB-specific antibody responses in serum samples from immunised mice on days 10, 25 and 75. Serum samples on day 0 were collected before the immunisation. <i>C,</i> Virus neutralizing capacity of antibody responses induced following immunisation with Ad-gBCMVpoly. Serum samples from these mice were pre-incubated with HCMV virus Ad169 and then these virus preps were used to infect MRC-5. Following overnight incubation virus infectivity was assessed using IE-1/IE-2 expression as outlined in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003256#s4" target="_blank">Material and Methods</a>” section. <i>D,</i> Avidity maturation of gB-specific antibody responses in Ad-gBCMVpoly immunised mice. <i>E,</i> Immunoglobulin subclass analysis of gB-specific antibody responses in HHD-2 vaccinated mice. Serum samples were collected from three different groups of mice on days 10, 25 and 75 post-immunisation. A minimum of five mice from each group were assessed for HCMV epitope-specific T cell reactivity and humoral immune responses. All statistical analyses were conducted using GraphPad Prism 4 software.