Pathology of the Abca12 mutant epidermis.

<p>Abca12 protein is detected in suprabasal keratinocytes in the granular and cornified cell layers of mutant and wild type epidermis (A). Keratinocytes in Abca12 mutant skin undergo premature differentiation highlighted by strong filaggrin expression in cells juxtaposed to K14⁺ basal keratinocytes (B) and increased co-expression of keratins 10 and 14 (white arrows), especially in basal cells (yellow arrows) (C). Increases in co-expression of K10 and K14 are significant at both E18.5 (p = 0.016) and at P1 (p = 4.44×10⁻⁶) (D). Samples are of E18.5 (A) or P1 (B, C) epidermis counterstained with DAPI. Scale bars = 30 µm.