Toxicity of LF proteins to mice.

<p>(A) LF-HMA and LF-A/St (100 µg) were injected in Balb/cJ mice in combination with 100 µg PA via the IP route and survival was monitored for 120 h. Each group contained n = 5 mice. (B) LF proteins (100 µg) were injected in Balb/cJ mice in combination with 100 µg PA via the IP route and survival was monitored for 120 h. Mouse numbers used in this experiment were as follows: LF-HMA (n = 9), LF-A (n = 9), LF-A/X (n = 5), LF-G/MyX (n = 3) and all other groups n = 4. (*) The animals in LF-G/MyX and LF-R/MyX groups exhibited substantial malaise starting at 24 h and throughout the experiment and were euthanized at 128 h to prevent suffering in accordance with approved animal protocols. (C) LF preparations (40 µg) were injected in Balb/cJ mice in combination with 40 µg PA via the IP route and survival was monitored for 120 h. Mouse numbers used in this experiment were as follows: LF-HMA (n = 14), LF-A (n = 9), LF-A/X (n = 5) and all other groups n = 4. (**) The surviving animals in these groups did not display any signs of malaise over the last 48 h of the experiment.