Analysis of β-catenin intracellular distribution in H460 cells and DSCs.

<p>Cells were fixed and incubated with Alexa Fluor® 488 phalloidin or with primary Abs against β-catenin and with secondary Alexa Fluor 488 conjugated Abs. Next cells were stained with Hoechst33342. Cell images were acquired using the Cellomics ArrayScan HCS Reader (20X objective) and analyzed using the Compartment Analysis BioApplication Software Module and the Target Activation BioApplication Software Module. <i>A, Images of H460 cells and DSCs immunofluorescently stained for β-catenin (A).</i> B, <i>An average fluorescence intensity of nuclear β-catenin in H460 (black line) and DSCs (grey line)</i>.C, <i>An average fluorescence intensity of cellular phosphor- β-catenin in H460 (black line) and DSCs (grey line). D, Cytoskeleton images of H460 cells and DSCs immunofluorescently stained for phalloidin and Hoechst33342.</i>