Effect of human antibodies on cleavage of SNAP-25 by BoNT/A in Neuro-2a cells.

<p>Neuro-2a cells were incubated with BoNT/A for 48 hours in the presence or absence of human antibody. (A) Cleavage of SNAP-25 was detected by immunoblotting of whole cell extracts. Removal of a 9 amino acid C-terminal fragment gives a band of reduced size (Cleaved) in addition to an intact SNAP-25 band (Uncleaved). Cells received either the antibodies indicated or culture medium without antibody (M). (B) The extent of SNAP-25 cleavage in the experiment shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003023#pone-0003023-g002" target="_blank">Figure 2A</a> was quantified by scanning densitometry. Values are reported normalized to the cell sample that contained BoNT/A but no antibody.