Codon changes were done at random and include those for numerous amino acids spanning the entire E7 gene.

<p>The location (amino acid number within the E7 gene) and identity of all codon changes is shown as well as the amino acids whose codons were changed. Genomes were built sequentially. Thus, E7/14 contains all the changes in E7/8 plus 6 unique to itself. E7/18 contains all E7/14 changes plus 4 unique to itself. E7/22 contains all of the previous changes plus 4 unique to itself.