RasGAP SH3 peptide aptamer binding specificity.
- Perayot Pamonsinlapatham (51926)
- Réda Hadj-Slimane (51927)
- Françoise Raynaud (51928)
- Marc Bickle (51929)
- Claudine Corneloup (51930)
- Audrey Barthelaix (51931)
- Yves Lepelletier (51932)
- Perrine Mercier (51933)
- Matthieu Schapira (51934)
- Jérôme Samson (51935)
- Anne-Laure Mathieu (51936)
- Nicolas Hugo (51937)
- Olivier Moncorgé (51938)
- Ivan Mikaelian (51939)
- Sylvie Dufour (51940)
- Christiane Garbay (51941)
- Pierre Colas (51942)
Publication date
February 2013
DOIAbstract
<p>(A) Protein domain mapping of the RasGAP protein. PPP: Proline-rich region; PH: Pleckstrin homology domain; C2: pKC-conserved region 2. (B) Sequence alignment of RasGAP, Nck, and Grb2 SH3 domains. Residues that are conserved between at least two proteins are highlighted in green. (C) Yeast two-hybrid interaction mating assay. LexA fusion proteins (“baits”) were expressed in EGY191α yeast containing a 8 <i>lexAop-lacZ</i> reporter gene. Control and selected peptide aptamers (fused to the B112 activation domain) were expressed in EGY42<u>a</u> yeast. Strains were mated and diploids were grown on X-gal-containing solid medium. 10M is a Cdk2 peptide aptamer and Control is a peptide aptamer randomly picked from the library.</p
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