Effect of aging and calorie restriction on gastrocnemius weights and non-heme iron content.

<p>(A) Gastrocnemius muscle weights from <i>ad libitum</i>-fed and calorie-restricted rats at 8, 18, 29, and 37 months of age. (B) Total non-heme iron content of gastrocnemius muscle was calculated from non-heme iron concentrations and muscle weights. (C) Non-heme iron concentrations were measured colorimetrically after acid digestion of tissue. Values are means±SEM (n = 8–9). ^a,b,c,dDifferent letters indicate values are significantly different (<i>p</i><0.05 by Tukey's Multiple Comparison Test).