<i>in vitro</i> binding of peptide aptamers to RasGAP SH3.
- Perayot Pamonsinlapatham (51926)
- Réda Hadj-Slimane (51927)
- Françoise Raynaud (51928)
- Marc Bickle (51929)
- Claudine Corneloup (51930)
- Audrey Barthelaix (51931)
- Yves Lepelletier (51932)
- Perrine Mercier (51933)
- Matthieu Schapira (51934)
- Jérôme Samson (51935)
- Anne-Laure Mathieu (51936)
- Nicolas Hugo (51937)
- Olivier Moncorgé (51938)
- Ivan Mikaelian (51939)
- Sylvie Dufour (51940)
- Christiane Garbay (51941)
- Pierre Colas (51942)
Publication date
February 2013
DOIAbstract
<p>Pull-down assay with recombinant purified RasGAP SH3. Recombinant purified 6xHis-RasGAP SH3 was added to GST-peptide aptamer fusion proteins, coupled to glutathion-sepharose beads. “Control” is a peptide aptamer that was selected against another target protein. Captured protein was revealed with an anti-His antibody. GST-aptamer fusions coupled to beads were stained with Coomassie blue.</p
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