Tetramer⁺ cells are more differentiated than the overall CD8 T-cell population, but there is little change in the relative proportions of the subpopulations in the second year post-diagnosis.

<p>A Scatter plots of the expression of CD27 and CD28 gated on all CD8 T-cells and on tetramer⁺ CD8 T-cells, and individual value plots of the percentages of cells that fell into each of the defined subpopulations within the overall CD8 and tetramer⁺ populations. B Scatter plots of the expression of CD27 and perforin gated on all CD8 T-cells and on tetramer⁺ CD8 T-cells, and individual value plots of the percentages of cells that fell into each of the defined subpopulations within the overall CD8 and tetramer⁺ populations. C Scatter plots of the expression of CD27 and granzyme A gated on all CD8 T-cells and on tetramer⁺ CD8 T-cells, and individual value plots of the percentages of cells that fell into each of the defined subpopulations within the overall CD8 and tetramer⁺ populations. Significant differences between sample times are indicated where present. Grey bars indicate means. Significant differences between tetramer⁺ and overall CD8 T-cell populations are indicated by *p<0.05, **p<0.005 or ***p<0.0005.