gL⁻ virions show altered glycoprotein and capsid distributions and gB conformation changes.

<p>A. NMuMG cells were exposed to wild-type or gL⁻ virions (2 h, 4°C, 3 p.f.u./cell) then washed ×3 in PBS and either fixed immediately (4°C) or first incubated (2 h, 37°C) to allow virion endocytosis (37°C). The cells were then permeabilized and stained for LAMP-1 plus MuHV-4 capsid or glycoprotein antigens as shown. Nuclei were counterstained with DAPI. Colocalization appears as yellow in the merged images. Equivalent data were obtained in 3 further experiments. B. MCCD epithelial cells were incubated with a range of different gL⁻ or gL⁺ viruses (2 h, 4°C, 3 p.f.u./cell), then washed ×3 with PBS and incubated further (2 h, 37°C) to allow virion endocytosis. The cells were then stained for gp150, an abundant virion glycoprotein, with mAb LS-B11 (IgG1) and for ORF65 capsid with mAb MG-12B8 (IgG2a). Nuclei were counterstained with DAPI.