BVLPs induced secretion of cytokines in DCs.

<p>DCs were incubated with BVLPs (10 µg/ml), heated BVLPs (10 µg/ml), LPS (10 µg/ml), Ac (culture supernatant of sf21 cells transfected with baculovirus expression vector pFastBac DUAL with <i>gfp</i> under the control of p10 promoter), or PBS. BVLPs + PMB or LPS + PMB: 10 µg/ml BVLPs or 10 µg/ml LPS was treated with 10 µg/ml polymyxin B at room temperature for 1 h before incubation with DCs. Following 16 h of treatment, supernatants were collected, and IL-6, IL-10 and TNF-α were analyzed using ELISA. Data are expressed as the mean±SD of triplicate samples.