Productive replication of HIV-1ΔVif in CEM-T4 cells.

<p>(A) Cell surface expression of HIV-1 receptors. HUT 78, H9, PM1, CEM-SS, CEM-T4, and A3.01 cells were stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CXCR4 antibodies (BD Biosciences). Expression of these surface molecules was determined by flow cytometry. (B) HIV-1 replication. Wild-type or <i>vif</i>-defective HIV-1 was produced from 293T cells after transfection with pNL4-3 or pNL4-3ΔVif. HUT 78, H9, PM1, CEM-SS, CEM-T4, and A3.01 cells were then infected with these viruses, and viral production was monitored daily using a p24^Gag ELISA for 8 d. Results shown are from 1 of 3 independent experiments. (C) A3G and A3F expression. Cell lysates were prepared from indicated human T cell lines, and the expression of A3G or A3F was determined by Western blotting.