Analysis of proliferation of thymocytes of Pbx1NT TG mice.

<p>Percentage of BrdU-positive DN1, DN2, DN3 and DN4 thymocytes after <i>in-vivo</i> BrdU staining (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002424#s2" target="_blank">Matherial and Methods</a>) is shown. The cells were from wild-type mice (open bars) or from Pbx1NT TG mice (black bars). (B) Cell cycle analysis of electronically gated DN2, DN3 and DN4 was performed with BrdU-flow kit (BD Biosciences, San Jose, CA) according to the manufacturer's recommendations. The cells were from wild-type mice (open bars) or from Pbx1ΝΤ TG mice (black bars).