Effect of FePro labeling on THP-1 cell ability to modulate T cells.

<p>The data depicts the levels of CD markers expression after 24 h of THP-1 cell-T cell co-culture. T cells were stained with FITC conjugated anti-CD3, PE/Cy5 conjugated CD4 and PE conjugated anti-CD8. Cells were gated based on the forward and side scatter characteristics (R1) and on the expression of CD3 marker (R2) (A). Flow cytometric histograms of T cells cultured without the presence of THP-1 cells (red line), T cells co-cultured with non-labeled THP-1 cells (blue line) and T cells co-cultured with FePro labeled THP-1 cells (green line) from representative experiment are shown. THP-1 cells induced modulation of T cell expression levels of CD4 and CD8 (B). Note the complete overlap of blue and green lines. At least 10,000 live-gated cells were analyzed for CD4 and CD8 expression.