Analysis of apoptosis of thymocytes of Pbx1NT TG mice.

<p>Purified DN thymocytes from Pbx1ΝΤ TG mice and their wild-type controls were stained with PE-conjugated anti-CD44, APC-conjugated anti-CD25, FITC-conjugated annexin V, and 7AAD prior to FACS analysis. The percentage of annexinV-positive 7AAD-negative DN3 and DN4 thymocytes <i>ex vivo</i> (0h) and after 24 hours of incubation at 37°C (24 h) is shown. The cells were from wild-type mice (open bars) or from Pbx1ΝΤ TG mice (black bars). Values that were significantly different are indicated as follows: *, P<0.05.