Retroviral-mediated expression of PPARγ2 rescues the impaired adipogenesis of FUS-DDIT3 MEFs.

<p>A) FUS-DDIT3 MEFs were infected with either control retroviral vector or one expressing PPARγ2 (pQCXIP-PPARγ2) and selected for 3 days with 2 μg/ml puromycin. Then, wild-type MEF, FUS-DDIT3 MEF and PPARγ2 expressing FUS-DDIT3-MEF were cultered up to confluence and grown in the presence of standard adipose differentiation induction medium. At day 8 after induction of adipocyte differentiation, cells were fixed and stained for neutral lipids with Oil-Red-O and the morphological differentiation is shown (the original magnification is ×20). This experiment was repeated three times using cells prepared from all lines and from different embryos and similar results were obtained. B) Analysis of the PPARγ2 protein by western-blot in FUS-DDIT3 MEFs infected with either a control retroviral vector (pQCXIP) or one expressing PPARγ2 (pQCXIP- PPARγ2) 4 days after infection.