Western-Blot Analysis Comparing USP Levels Derived from Different Transgenic Stocks.

<p>Protein extracts were prepared from salivary glands of three different crosses at the wandering-L3 stage. Flies of the tester stock (<i>sgGal4</i>; <i>GFP.nls</i>; <i>glueRED</i>) were crossed to a control to ascertain the normal amount of USP protein present in third-instar glands (<i>w¹¹¹⁸</i>). The same tester stock was crossed to a line in which RNAi against <i>USP</i> could be induced in the salivary glands (<i>USPi</i>), and to a line in which wildtype <i>USP</i> could be overexpressed (<i>USP+</i>). The blot was cut, and one part was incubated with antibody against USP and the other was incubated with α-Tubulin as a loading/blotting control.