DNA sequencing chromatograms showing the mutations of affected members in Family A and Family B.

<p>In Family A, male patients show c.947A>G transition that is predicted to result in a p.D316G substitution in EDA and female carriers have A/G heterozygosity at the same position. In Family B, male patients show c.1013C>T transition that is predicted to result in a p.T338M substitution in EDA and female carriers are C/T heterozygous at the same position. Patient: male patients (hemizygous), carrier: female carriers (heterozygous), normal: normal individuals (no mutation).