M867 inhibits apoptosis and reduces clonogenic survival of H460 lung cancer cells.

<p>(A) H460 NSCLC cells were treated with DMSO control, M867 (dose range from 1.4 to 10 nM, for 24hrs) with radiation. After 8 d, colonies were stained and the scored colonies were graphed. Survival curves for H460 cells±M867 treatment. Points, mean; bars, SD (<i>P</i> = 0.007). Shown are the mean +/− the standard deviation of three separate repeated experiments. (B) Annexin-V assay showing the level of apoptosis in H460 cells treated by either control, 100nM of M867 for 2h, radiation (5, 10 or 20 Gy), and both modality. This was done in triplicate and the average of the three counts was calculated. Columns, average; bars, SD.