RT-PCR analysis of RNA isolated from liver (lanes 1–3, 10–12), spleen (lanes 4–6, 13–15) and pulmonary (lanes 7–9, 16–18) tissues of mouse injected with agrobacteria harboring CMV promoter-based binary constructs with the GFP (lanes 1–9) or GFPi gene (lanes 10–18).

<p>C1, control 1–RNA isolated from a mouse injected with agrobacteria carrying an empty vector; C2, C3 controls 2 and 3–RNA isolated from HeLa cells transfected with the CMV-based constructs containing the GFP or GFPi gene, respectively. Top panel, GFP-specific RT-PCR products; bottom panel, actin-specific RT-PCR products used as a loading control.