Infection-induced apoptosis of MZB cells. CD21^HighCD23^Low cells were gated and analyzed for 7AAD and Annexin V staining.

<p>The percentage of positive cells was calculated in MZ B cells from naïve mice, as well as from <i>T. brucei</i> AnTat 1.1E infected mice on days 4, 7 and 10 (A). CD21^HighCD23^Low cells were FACS sorted on day 7, lyzed and analyzed in Western Blot with an anti-caspase 3 antibody, showing bands at 32 kD (pro-caspase 3), and cleaved forms 17 kD and 12 kD (B). RT-PCR for Casp3, BAFF-R, and Bcl-2 was performed on CD19⁺ spleen cells isolated from naïve mice as well as from <i>T. brucei-</i>infected mice on day 4 and day 7 (C). Results present the means of 3 mice per time point ±SD. One of three representative experiments is shown.