Immunoreactivity of STF2.HA1-1, STF2.HA1-2 and STF2.HA1-3 in ELISA.
- Langzhou Song (46797)
- Valerian Nakaar (46798)
- Uma Kavita (46799)
- Albert Price (46800)
- Jim Huleatt (46801)
- Jie Tang (46802)
- Andrea Jacobs (46803)
- Ge Liu (46804)
- Yan Huang (46805)
- Priyanka Desai (46806)
- Gail Maksymiuk (46807)
- Virginia Takahashi (46808)
- Scott Umlauf (46809)
- Lucia Reiserova (46810)
- Rodney Bell (46811)
- Hong Li (20183)
- Yi Zhang (9093)
- William F. McDonald (46812)
- T. J. Powell (46813)
- Lynda Tussey (10458)
Publication date
February 2013
DOIAbstract
<p>ELISA plates were coated with the 0.2 µg/well of indicated STF2 fusion proteins, PR8 virus or the full length PR8 HA0 ectodomain expressed in Hi5 cells. Plates were probed with either naïve or PR8 convalescent sera at indicated dilution. Following incubation with HRP-conjugated goat anti-mouse IgG, plates were developed with UltraTMB substrate. Results reflect the delta value of OD<sub>450</sub> (Convalescence-Naïve) of samples performed in duplicate. Naïve values (data not shown) were below 0.02.</p
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