Characteristics of mouse macrophages.

<p>A. Cholesterol efflux from cultured peritoneal macrophages. Cells were loaded with radioactive cholesterol as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002255#s2" target="_blank">Methods</a>. Subsequently, culture medium with human HDL as an acceptor was added. Left panel: Radioactivity was measured in aliquots from the cultured medium taken at 0, 1, 2, 4, and 6 hours. Circles: macrophages from C57BL/6J mice, squares: macrophages from huPLTP^tg/tg mice. Differences were not statistically significant. B. PLTP activity measured in the medium of macrophages cultured for 24 hours in the absence (white bars) or presence (black bars) of 100 ng/mL LPS. Cells from 6 mice were used per group. C. PLTP activity measured in plasma samples from mice treated with thioglycolate. N = 6 mice per group. ** <i>p</i><0.005, *** <i>p</i><0.001 versus cells without LPS or thioglycolate (same genotype), ## <i>p</i><0.005, ### <i>p</i><0.001 versus PLPT^wt/wt (same culture medium, with or without LPS or thioglycolate).