Role of the G protein/ROCK/moesin pathway for ER− breast cancer cell migration.

<p>ER− MDA-MB-468 cells were exposed to 10 nM E2, in the presence or absence of the ER antagonist ICI 182,720 (ICI; 1 µM), of the G protein inhibitor PTX (100 ng/ml), or of the ROCK-2 inhibitor, Y-27632 (Y; 10 µM) or of the selective estrogen receptor modulator (SERM) tamoxifen (TAM, 10 nM). Breast cancer cells were scraped from the culture dish and the mean migration distance of the cells from the starting line was assayed after 48 h and expressed as mean±SD. Representative images are shown and the mean±SD of migration are shown in the bar graphs. * = p<0.05 vs. control.