Casp8p41 upregulation of HIV LTR is NF-κB dependent.

<p>(A) 293T cells were transfected with empty vector control, Casp8FL or Casp8p41 along with HIV-LTR-Luc or with HIV-LTR-Luc with the KB site deleted (HIV-LTR-ΔKB-Luc) as indicated. Results of three independent experiments expressed as fold increase relative to control, normalized to Renilla, +/− SD. Results expressed as luciferase expression per Renilla. *p = <0.05 compared to HIV LTR. (B) 293T cells were transfected with empty vector control HA-Casp8FL or HA-Casp8p41 and analyzed by EMSA in the presence or absence of anti-p50 or anti-p65 antibodies. As a control for the antibodies EMSA's were performed in cells transfected with p50/p65 in the presence or absence of anti-p50 or p65 antibodies. The level of Casp8FL and Casp8p41 expression were determined in parallel.